FIG 5.

C19ORF66 knockdown results in higher viral production yield and higher viral gene expression levels. (A) Diagram depicting the supernatant transfer assay. (B) Supernatant transfer assay was used as a proxy for virion production and performed as described in panel A. Infection of 293T cells was monitored by imaging GFP on a fluorescence microscope. (C) Quantification of GFP-positive cells. Values represent four independent views of the infected cells. (D to F) Total RNA was extracted from iSLK.219 cells treated with siRNAs targeting C19ORF66 (or control nontarget siRNAs) for 48 h and reactivated for the indicated times. RNA was then subjected to RT-qPCR to quantify expression of the indicated viral genes. n.s., not significant; **, P < 0.01; ***, P < 0.001.