FIG 6.

SAHA affects late gene expression through E1A-independent mechanisms. (A) Schematic diagram of the Ad-CMV/E1A construct (not drawn to scale). The E1 promoter was replaced with the CMV promoter to allow high level expression of the E1 proteins even in the presence of SAHA. (B) A549 cells were infected with Ad-late/RFP or Ad-CMV/E1A (MOI of 10) and treated with vehicle or 10 μM SAHA. Cell lysates were collected for immunoblot analysis of E1A and fiber at 24 h postinfection. (C) 293 cells were infected with Ad-late/RFP (MOI of 10) and treated with 10 μM SAHA for the indicated time points. (D) 293 cells were infected with Ad(E1^–)-late/RFP (MOI of 10) and treated with SAHA for 24 h. Neither forced expression of E1A nor infection in the presence of existing E1A from 293 cells fully rescued fiber expression in SAHA-treated cells.