FIG 7.

SAHA-induced increase in p21 is not responsible for the impairment of HAdV replication. (A) p21 levels were detected by immunoblot analysis in both infected (Ad-late/RFP, MOI of 10) and uninfected A549 cells treated with vehicle or 10 μM SAHA. SAHA treatment increases p21 expression in both infected and uninfected cells, while infection with Ad-late/RFP alone decreases expression. (B) Cells were transfected with control siRNA or siRNA specific to the CDKN1A transcript for 48 h to transiently knock down cellular p21. (C) These cells were infected with Ad-late/RFP and treated with vehicle or SAHA. Loss of p21 did not rescue late gene expression at 24 hpi following SAHA treatment.