FIG 3.
Antigenic profile of cell surface DS and SOS Envs. (A and B) The binding of antibodies to the HIV-1JR-FL Env variants expressed on the surface of HOS cells was measured by a cell-based ELISA. (A) Cytoplasmic tail-deleted EnvΔ712 variants. (B) Full-length Env variants. The RLU value associated with the binding of soluble CD4 (sCD4) or the indicated antibody was normalized relative to the detection of the 2G12 antibody. The experiments were conducted in the absence of a reducing agent (left) or in the presence of 1 mM DTT (right). Data represent the averages and standard errors of the means from at least three independent experiments, each performed in quadruplicate. Statistical significance was evaluated using multiple-comparison one-way analysis of variance (ANOVA) or a Kruskal-Wallis test; P values near the level of significance are reported as numerals, and significant P values are indicated (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001).