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Analysis of plaque size and efficiency of plating at permissive and nonpermissive temperatures. (A) Representative plaque assays at 32 and 40°C for icWT, tsNC11, and engineered mutant viruses. The dilution of the viral stock is indicated and selected to visualize ca. 20 to 50 plaques per plate. (B) EOP data. EOP = average titer at 40°C/average titer at 32°C.
