FIG 1.

Only CPXV CP77 can bind Chinese hamster SAMD9L, while only VACV K1 can bind Chinese hamster SAMD9. 293FT cells were transfected with a plasmid expressing Flag-tagged chSAMD9L (A) or chSAMD9 (B) and infected with a panel of vK1⁻C7⁻-derived VACVs that expressed different V5-tagged viral proteins. A virus expressing VACV-A6, a viral protein involved in viral assembly, was used as a negative control. The cell lysates were immunoprecipitated with anti-V5 antibody. Epitope-tagged proteins in the cell lysate and precipitate were detected with anti-Flag or anti-V5 antibody in Western blotting (WB). The heavy and light chains of the precipitated antibody (Ig HC and LC) served as loading controls. *, V5-tagged proteins.