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The block in viral protein synthesis in CHO cells can be abolished by knocking out chSAMD9L. BSC40 cells and the parental and ΔSAMD9L (Δ9L, 2F clone) CHO cells were infected with VACVs that expressed a luciferase reporter under the control of either the synthetic early/late (E/L) promoter or the late p11 promoter in the presence or absence of AraC. The cells were lysed after either 8 h (A) or 24 h (B) of infection, and luciferase (LUC) activities were measured.
