FIG 4.

Expression of chSAMD9L in human cells is sufficient for recapitulating the poxvirus restriction property of CHO cells. (A) A stable human BT20 cell line with inducible expression of chSAMD9L was established via lentiviral transduction. The cell line (i-chSAMD9L) was cultured with medium containing the indicated concentration of doxycycline (Dox). The levels of chSAMD9L and the control HSP70 protein in the cell lysates were determined by Western blotting. (B) The i-chSAMD9L cells were untreated or treated with 1 μg/ml Dox. The cells were then infected with the panel of vK1⁻C7⁻-derived VACVs. Viral titers at 0 and 24 hpi were measured by plaque assay in Vero cells.