FIG 5.

CP77 is an inhibitor of hSAMD9, hSAMD9L, and mSAMD9L. (A) 293FT cells were transfected with a plasmid expressing Flag-tagged hSAMD9, hSAMD9L, or mSAMD9L and infected with VACVs that expressed different V5-tagged viral proteins. Immunoprecipitation and detection were performed as described for Fig. 1 (B) HeLa, ΔhSAMD9 HeLa, or 3T3 cells were infected with the indicated viruses, and viral growth was determined by measuring viral titers at 0 and 48 hpi. ΔhSAMD9 HeLa cell were pretreated with IFN-β (100 U/ml) for 24 h prior to the infection.