FIG 6.

K1 and CP77 target a common internal region of SAMD9&L. (A) Schematics of the predicted SAMD9&L domain architecture and the SAMD9&L truncation with only the NTPase-TPR domain. (B and C) 293FT cells were transfected with a plasmid expressing the putative NTPase and TPR domains of SAMD9&L (aa 607 to 1172 for hSAMD9, aa 598 to 1172 for mSAMD9L, and aa 594 to 1172 for chSAMD9L) and infected with VACV expressing C7, K1, or CP77. Coimmunoprecipitation and Western blotting were performed as described for Fig. 1 The two K1 substitution mutations (S2C-2 or S1-mut6) were previously shown to disrupt K1 host range function in human cells (20).