FIG 7.

The N-terminal 382 aa of CP77 are sufficient for binding to chSAMD9L. (A) Schematics of different CP77 constructs. Ankyrin repeats are shown as boxes and numbered. The C-terminal F-box is indicated by “F.” ΔC, deletion of aa 383 to 656 (maintaining the N-terminal seven ankyrin repeats); Δ5, deletion of aa 235 to 266 (ankyrin repeat 5). (B) CHO cells were infected with vK1⁻C7⁻-derived VACV expressing either WT or mutated CP77. Viral growth was determined as described for Fig. 2. (C) Full-length chSAMD9L and aa 594 to 1172 of chSAMD9L were subjected to coimmunoprecipitation with either WT or mutated CP77 as described for Fig. 1.