FIG 1.

IFN-β, IFIT-1, and CXCL10 expression in porcine alveolar macrophages infected by ASFV NH/P68 or Armenia/07 strains. Porcine alveolar macrophages were mock infected or infected with NH/P68 or Armenia/07 strain (4 PFU/cell). Cells were collected at 4, 8, or 16 hpi. qPCR analyses of IFN-β (A), IFIT-1 (C), and CXCL10 (D) and of ASFV viral protein p32 (E) mRNAs were performed. (B) Supernatants were collected at 4, 8, or 16 hpi, and the concentration of IFN-β was determined by ELISA. (F) Cells were lysed in RIPA buffer at 4, 8, and 16 hpi, and lysates were separated by 7 to 20% SDS-PAGE, followed by immunoblotting with anti-ASFV p32 (viral early p32) and anti-actin antibodies. The data are means ± the standard errors of the mean (SEM; n = 3). Data were statistically analyzed by using a Student t test (*, P < 0.05; **, P < 0.01; ***, P < 0.001).