FIG 4.

The activation of cGAS-STING by the attenuated strain NH/P68 promotes the translocation to the nucleus of p-IRF3. (A) Scheme of the biochemical fractionation method. See Materials and Methods for details. (B) Porcine alveolar macrophages were mock infected or infected with NH/P68 or Armenia/07 strain (2 PFU/cell). At 8 hpi the cells were collected, and chromatin fractionation was performed. The WCE, cytoplasmic fraction (S2), chromatin fraction (P3), and soluble nuclear fraction (S3) were separated by 7 to 20% SDS-PAGE, followed by immunoblotting with anti-p-IRF3, anti-β1-laminin, anti-H3 (histone 3), anti-ASFV p32 (viral early p32), and anti-actin antibodies.