FIG 6.

Armenia/07 blocks the phosphorylation of STING and TBK1 mediated by cGAMP. (A) Porcine alveolar macrophages were mock infected or infected with Armenia/07 strain with 2, 4, or 8 PFU/cell. At 5 hpi, the cells were untreated or treated with 10 μg/ml of cGAMP. At 2 h after cGAMP treatment, the cells were collected. Numbers below the bands indicate the relative levels of p32 viral protein. Densitometry was performed using ImageJ. (B) Porcine alveolar macrophages were mock infected or infected with NH/P68 or Armenia/07 strain (2 PFU/cell) and untreated or treated with 40 μg/ml of cytosine arabinoside (AraC). At 14 hpi, the cells were untreated or treated with 10 μg/ml of cGAMP. At 2 h after cGAMP treatment, the cells were collected. (A and B) Cells were lysed in RIPA buffer, and lysates were separated by 7 to 20% SDS-PAGE, followed by immunoblotting with anti-p-STING, anti-p-TBK1, anti-ASFV p32 (viral early p32 protein), and anti-actin antibodies.