FIG 7.

STING locates at perinuclear clusters in porcine alveolar macrophages during NH/P68 but not during Armenia/07 infection. (A) Porcine alveolar macrophages were mock infected or infected with NH/P68 or Armenia/07 (5 PFU/cell) or treated with cGAMP (20 μg/ml). At 6 hpi or 1 h after cGAMP treatment, the cells were fixed and stained with DAPI and anti-STING antibody and then examined by using a confocal microscope. Arrows indicate punctuated perinuclear structures. An arrowhead indicates viral factories. (B) Quantitative analysis of cells with punctate pattern or diffused signal is presented. The percentage of cell number was calculated among 75 cells under each condition.