FIG 9.

Quantification of STING colocalization with AP1 at very early or early time points after NH/P68 and Armenia/07 infections. (A) Porcine alveolar macrophages were mock infected or infected with NH/P68 or Armenia/07 strain (2 PFU/cell) or treated with cGAMP (20 μg/ml). At 1 and 6 hpi or 1 h after cGAMP treatment, the cells were fixed and stained with DAPI (blue), anti-STING (green), and anti-AP1 (red) antibodies and examined by using a confocal microscope. Cells where STING exhibit a punctuate pattern are highlighted (arrows). (B and C) Quantification of fraction of STING overlapping AP1 (Mander’s coefficient) for each condition (B) and only for cells with a STING punctuated pattern (arrows) at 1 hpi and cGAMP-treated cells (C). The data are means ± the SD (n = 50). Data were statistically analyzed by using a Student t test (*, P < 0.05; **, P < 0.01; ***, P < 0.001).