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. Author manuscript; available in PMC: 2020 Jul 15.
Published in final edited form as: Mol Cell Endocrinol. 2019 May 14;492:110445. doi: 10.1016/j.mce.2019.05.004

Fig. 3. Generation and characterization of γ2A stable cells expressing GHR, PRLR, GHR(PRLR-S2), and PRLR(GHR-S2).

Fig. 3

A, Schematic diagram of domain-swapped human GHR/PRLR chimeras. GHR is in white. PRLR is in gray. GHR(PRLR-S2) is GHR with its S2 replaced with that of PRLR. PRLR(GHR-S2) is PRLR with its S2 replaced with that of GHR. Detergent extracts of γ2A-JAK2 cells stably expressing each receptor were resolved by SDS-PAGE and immunoblotted with antibody specific to the cytoplasmic domain of each receptor. B,C GH- and PRL-induced signaling in γ2A stable cells expressing GHR, PRLR, GHR(PRLR-S2), and PRLR(GHR-S2). Serum-starved cells were treated with vehicle (−), GH, or PRL at 500 ng/ml for 10 min. Detergent extracts were resolved by SDS-PAGE under reducing conditions and sequentially immunoblotted for pJAK2, total JAK2, pSTAT5, and STAT5. Detergent extracts were resolved on a separate SDS-PAGE and immunoblotted by anti-GHR or anti-PRLR to verify receptor expression. The data shown are representative of three such experiments.