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. 2019 Feb 20;15(7):1296–1308. doi: 10.1080/15548627.2019.1580509

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© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Mitochondrial architecture, mitophagy and autophagy in the adult lens. (a) Schematic of the mouse eye depicting anatomical location of the lens in light blue. Boxed area is shown in panel (b) and gray arrow represents light path. (b) Overview photomicrograph of mito-QC eye section depicting the lens; LE, lens epithelium; Eq, equatorial region; OFZ, organelle-free zone; CB, ciliary body. Gray arrow, as in panel A. Scale bar: 100 μm. (c) Quantification of mCherry-only puncta from mito-QC (mitophagy) or mCherry-GFP-LC3 (autophagy) lens sections. Data points represent means from individual animals ±SEM (***P < 0.0001). (d) Upper left panel shows optical section of the adult lens equatorial region from a mito-QC mouse, below is magnified area of lens epithelium showing small spherical mitochondria and minimal mitophagy, with examples of mitolysosomes marked by arrows. Panel on right shows maximum Z-projection of lens optical slices. (e) Upper left panel shows optical section of the adult lens equatorial region from a mCherry-GFP-LC3 mouse, below is magnified area of lens epithelium with examples of autolysosomes marked by arrows and autophagosomes by arrowheads. Panel on right shows Z-projection of lens optical slices. (f) Isosurface render detailing mitochondrial network in mito-QC with lens epithelium on the left side of the panel (lateral to medial = left to right). Arrows denote examples of elongated mitochondria and arrowheads that of smaller solitary mitochondria. (g) Optical sections from E16.5 eyes detailing equatorial region (Eq), hyaloid vessels (HV) are also marked. Magnified epithelium sections are shown on the right, with arrows showing examples of mitolysosomes (top panel) and autolysosomes (bottom panel). Arrowheads mark autophagosomes. (h) Quantification of mCherry-only puncta from mito-QC (mitophagy) or mCherry-GFP-LC3 (autophagy) E16.5 lens sections. Data points represent means from individual animals ±SEM (***P < 0.0001). Scale bars (panels D-G): 10 μm.