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. 2019 Feb 22;15(7):1182–1198. doi: 10.1080/15548627.2019.1580095

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Figure 3. — PRKN is not required for mitophagy in CPCs. (a) Representative western blots of PRKN and GAPDH in mouse CPCs and adult hearts. (b) Real-time PCR analysis of Prkn transcript levels in CPCs and heart tissue (n = 3). (c) Representative western blots of TIMM23 and ACTA1 in WT and prkn^−/- CPCs after treatment with 25 μM FCCP for 24 h. (d) Quantification of TIMM23:ACTA1 in WT and prkn^−/- CPCs (n = 3). (e) The number and percentage of cells with mRNA detected by single-cell RNA sequencing for Prkn and mitophagy genes in mouse CPCs at passage 0 (fresh) or passage 5 (cultured). Violin plots display gene expression of mitophagy genes in mouse CPCs. (f) The number and percentage of cells with mRNA detected by single-cell RNA sequencing for PRKN and mitophagy receptors in human CPCs at passage 5 (cultured). Violin plots display gene expression of mitophagy genes in human CPCs. Data are mean ± SEM. ***p < 0.001; n.s., not significant.