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. 2019 Feb 20;15(7):1214–1233. doi: 10.1080/15548627.2019.1580103

Figure 8.

Figure 8.

Reducing endosome levels rescues the lysosomal and autophagic defects. (a) Control or VAPA/B siRNA-transfected HEK293T cells were treated with DMSO or PI4KIIIbeta-IN-10 for 4 h. WB probed for TFEB and CTSB. (b) Control or VAPA/B siRNA-transfected HEK293T cells are treated with DMSO or PI4KIIIbeta-IN-10 for 18 h and treated with bafilomycin A1 for 4 h. WBs probed with LC3. (c) Immunofluorescence staining of ref(2)P/SQSTM1 in muscles of Vap33∆20 mutant and Vap33∆20; Rab5KO/+ larvae. Bottom: Quantification of the ref(2)P intensity. (d) Immunofluorescence staining of Rab7 and ref(2)P in muscles of Vap33∆20 mutant and Vap33∆20; Rab7KO/+ larvae. Bottom: Quantification of the Rab7 intensity and number of ref(2)P puncta. (e) Crawling assay of control, Vap33∆20 mutant, Vap33∆20 mutant treated with PI4KIIIbeta-IN-10 (5 nM) and Vap33∆20; Rab7KO/+ larvae. Ten to 20 animals are tested for each genotype/treatment.