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. 2019 Mar 20;15(8):1391–1406. doi: 10.1080/15548627.2019.1582973

Figure 4.

Figure 4.

Radiation-induced activation profile of specific UPR pathways. (a) Immunoblot analysis of radiation-induced changes in specific UPR branch proteins at the early time point (0–1 h post-irradiation); blots were probed with HSPA5, ERN1, ATF6, p-EIF2AK3 and total EIF2AK3 specific antibodies. The time point of 0 h was taken immediately after radiation. (b) Western blot analysis of ERN1, ATF6 and p-EIF2AK3 levels at 12 and 24 h post-irradiation. (c) Immunoblot analysis of radiation-induced changes in EIF2AK3 phosphorylation and autophagy levels (LC3-II), in the presence of the EIF2AK3 inhibitor GSK2606414 at 12 and 24 h post-irradiation. The graph shows quantification of band intensities (relative to ACTB). (d) Immunoblot analysis of radiation-induced changes in the cleavage of XBP1 and autophagy levels (LC3-II), in the presence of the ERN1 inhibitor DBSA at 12 and 24 h post-irradiation. The graph shows quantification of band intensities (relative to ACTB) from 3 independent experiments.