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Rate of eGFP-Map1lc3b-positive puncta accumulation is higher in rapamycin-treated samples. Rates of eGFP-Map1lc3b-positive puncta accumulation in the optic tectum of 3-dpf larvae that underwent indicated treatment for 24 h over 60 min were obtained via addition of BafA under the indicated drug treatment (see Materials and Methods). Mean ± s.e.m. shown; n ≥ 12 for each group. **p < 0.01, p > 0.05 (n.s.), one-way ANOVA with Dunnett’s multiple comparison analysis (GraphPad Prism) .
