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. 2019 Mar 1;15(8):1322–1332. doi: 10.1080/15548627.2019.1580511

Figure 5.

Figure 5.

Isradipine does not induce autophagosome formation nor alter autophagic flux. (a) Representative maximum confocal projections of the optic tectum regions of 3-dpf larvae that underwent indicated treatment for 24 h. Scale bars: 10 μm. (b) Quantification of the numbers of eGFP-Map1lc3b-positive puncta in the optic tectum of 3-dpf larvae that underwent indicated treatment for 24 h; mean ± s.e.m. shown; n ≥ 12 for each group. ****p < 0.0001, *p < 0.05, p > 0.05 (n.s.), one-way ANOVA with Tukey’s multiple comparison analysis (GraphPad Prism). (c) Quantification of rate of eGFP-Map1lc3b-positive puncta accumulation in the optic tectum of 3-dpf larvae that underwent indicated treatment for 24 h over 60 min; mean ± s.e.m. shown; n ≥ 11 for each group. p > 0.05 (n.s.), two-tailed unpaired t test (GraphPad Prism) .