Fig. 2. Generation of an iPSC line from an AD patient harboring the APP-V715M mutation. (A) Established iPSC lines from the APP-V715M patient showing the expression of pluripotent stem cell markers, including OCT4 (red), SOX2 (green), SSEA4 (red) and TRA-1-81 (red). (B) Immunocytochemistry showing the potential of iPSC line to form three germ layers, including ectoderm (TUJ1, green), mesoderm (SMA, green), and endoderm (AFP, red). Scale bar: 100 µm. (C) Genomic DNA sequences showing the presence of the heterozygous V715M mutation (GTG to ATG) in the APP gene of the APP-V715M iPSC line. (D) Reverse-transcription PCR analysis showing the absence of integration of the Sendai virus vectors. (E) Karyotype analysis of the APP-V715M iPSC line. (F) In vivo teratoma analysis showing the formation of all three germ layers: Tuj1-positive neurons (ectoderm), cartilage (mesoderm) and gut-like epithelium (endoderm). Scale bar: 100 µm.