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Experimental validation of circRNAs. (A) qRT-PCR analysis of seven circRNAs with different expression patterns in response to SUS. β-actin is the internal reference. N = 3, ∗p < 0.05, ∗∗p < 0.01. (B) Outward-facing primers and inward-facing primers were designed to compare total and RNase R-treated RNAs from coelomocytes by gel electrophoresis. Linear mRNA forms are depleted by RNase R, whereas circular ones are resistant. CircRNA: AJAPscaffold1149:136110| 200955; linear RNA: ficolin.
