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. 2019 Jul 2;10:1496. doi: 10.3389/fmicb.2019.01496

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Copyright © 2019 Wang, Liu, Liu, Niu and Deng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Identification of the key nucleotide sequence required for the inhibition of LLO production by luteolin. The hly gene was truncated and cloned into the pET21a⁺ vector. Truncated LLO was synthesized in vitro using a S30 T7 High-Yield Protein Expression System based on constructed expression plasmids in the presence of the indicated concentrations of luteolin. Then, the total production of truncated LLO in the protein expression system was determined using Western blotting. (A–E) The production of truncated LLO from the indicated nucleotide sequence.