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. 2019 Jul 2;13:281. doi: 10.3389/fncel.2019.00281

FIGURE 4.

FIGURE 4

Addition of TG2 to DRGN-OPC co-cultures increases myelination. (A,B) Exogenous guinea pig TG2 (gpTG2, 25 μg/ml every 3 days from day 2 in vitro for 11 days) was added to dorsal root ganglion neurons-oligodendrocyte progenitor cell (DRGN-OPC) co-cultures. Myelination was measured as percentage of myelinated axons, visualized with myelin basic protein (MBP, myelin marker, green) of total neurofilament (NF, red)-positive axons 14 days in co-culture. Nuclei are visualized with DAPI (blue). Representative images are shown in A, and the relative percentage of myelinated axons in gpTG2-treated DRGN-OPC co-cultures to vehicle-treated (CTRL) co-cultures is shown in B (n = 3). (C,D) Wild-type human TG2 (hTG2) was lentivirally (LV TG2) overexpressed in OPCs, and plated onto DRGNs. The extent of myelination was analyzed at 14 days in co-culture. Representative images are shown in C, and the relative percentage of myelinated axons in DRGN-OPC co-cultures with hTG2-expressing OPCs to MOCK OPCs is shown in D (n = 3). Data are shown as mean + SEM, calculated as average percentage compared to vehicle-treated (B, CTRL) or MOCK-transduced OPCs (D), which were set to 100% in each independent experiment. Statistical analyses were performed using a one-sample t-test (*p < 0.05). Scale bar is 100 μm.