Fig. 4. ChC^ILpre-exos-mediated production of mature IL-1βis partially dependent on the increase of mitoROS.

a PMA-induced THP-1 cells were treated with pChC^NCpre-exos or pChC^ILpre-exos in the presence or absence of LPS and then the level of mitochondrial ROS (mitoROS) were detected using MitoSox fluorescent probe. b PMA-induced THP-1 cells were treated with pChC^NCpre-exos or pChC^ILpre-exos in the presence of LPS for 24 h, combined with 100 nM Baf A1 or 20 μM CQ for aftermost 3 h. Then the level of mitoROS were measured. c PMA-induced THP-1 cells were pre-treated with 20 μM mito-TEMPO (a mitochondrial-specific ROS scavenger), followed with pChC^NCpre-exos or pChC^ILpre-exos in the presence of LPS for 24 h. Then the supernatant IL-1β were measured using ELISA assay. d PMA-induced THP-1 cells were treated with swChC^NCpre-exos or swChC^ILpre-exos in the presence or absence of LPS and then the level of mitoROS were detected. e PMA-induced THP-1 cells were treated with swChC^NCpre-exos or swChC^ILpre-exos in the presence of LPS for 24 h, combined with 100 nM Baf A1 or 20 μM CQ for aftermost 3 h. Then the level of mitoROS were measured. f PMA-induced THP-1 cells were pre-treated with 20 μM mito-TEMPO, followed with swChC^NCpre-exos or swChC^ILpre-exos in the presence of LPS for 24 h. Then the supernatant IL-1β was measured using ELISA assay. ANOVA with Bonferroni’s multiple comparison test was used, *p < 0.05; ns, no significance