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. 2019 Jul 2;10:1486. doi: 10.3389/fmicb.2019.01486

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Copyright © 2019 O’Brien, Almaraz, Foster, Hill, Huber, King, Langford, Lowe, Mickan, Miller, Moore, Mathes, Gleeson and Leopold.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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KEGG orthologs (KOs) involved in N-fixation via (A) Nif-specific regulatory protein, (B,H) Nitrogen fixation protein NifB and NifX, (C,F,G) Nitrogenase molybdenum-iron protein alpha chain, beta chain, and NifN, (D) Nitrogenase molybdenum-cofactor synthesis protein NifE, (E) Nitrogenase iron protein NifH, with significantly higher abundances in the plateau and bottom sections relative to all others (top, near top, and mid) on T140. KO abundances were calculated using PICRUSt and visualized in STAMP (minimum effect size = 0.7, Benjamini–Hochberg multiple test-corrected q-value < 0.05).