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. 2019 Jul 2;10:1500. doi: 10.3389/fimmu.2019.01500

Figure 1.

Figure 1

A representative SPOTi image comparing human RNases activity on Mycobacterium aurum infected macrophages. RAW 264.7 macrophages were infected with M. aurum at 10:1 MOI for 3 h at 37°C. The culture was washed with RPMI-1640 thrice and incubated with different concentrations of the proteins in RPMI-1640 complete medium for 4 h (Left) or 24 h (Right). Macrophages were washed twice with RPMI-1640 and lysed. Then, an aliquot was spotted onto wells of a 24-well plate containing MB7H10/OADC/agar and incubated at 37°C for 4–5 days to determine intracellular survival.