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. 2019 Jul 2;10:1500. doi: 10.3389/fimmu.2019.01500

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Copyright © 2019 Lu, Arranz-Trullén, Prats-Ejarque, Pulido, Bhakta and Boix.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Both RNase3 and the RNase-H15A active site mutant induce autophagy in macrophages. RAW 264.7 mouse macrophages were infected with M. aurum and treated with 10 μM of protein (RNase3 and RNase3-H15A). Real-time qPCR measured the relative expression of BECN1 (A) and ATG5 (B) genes normalized by the housekeeping gene β-actin. Results are shown from 3 independent experiments (mean ± SD); (C) Immunoblot of LC3-processing and the housekeeping GAPDH control. Results are shown from 3 independent experiments (mean ± SD). *Statistically significant differences compared with the control sample are indicated (p value < 0.05).