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. 2019 Jul 2;10:1500. doi: 10.3389/fimmu.2019.01500

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Copyright © 2019 Lu, Arranz-Trullén, Prats-Ejarque, Pulido, Bhakta and Boix.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Autophagy induction by RNase3 contributes to its antimycobacterial activity against M. aurum. Mouse macrophages were infected with M. aurum and treated with 5 μM of RNase3 and/or 100 nM of rapamycin, and/or 100 nM of bafilomycin A1, and/or 5 mM of 3 methyladenine for 24 h. Real-time qPCR measured the relative expression of BECN1 (A) and ATG5 (B) genes normalized by the housekeeping gene β-actin. Results are shown from 3 independent experiments (mean ± SD); (C) A representative immunoblot of LC3 and housekeeping GAPDH control; (D) Intracellular mycobacterial viability was determined based on the number of CFUs. Relative percentages to control samples are indicated. Results are shown from 3 independent experiments (mean ± SD). *Significant differences between pairs is indicated (p value < 0.05).