Figure 8.
Comparative analysis by immunofluorescence microscopy of LC3 accumulation following RNase3 treatment in the absence and presence of Bafilomycin A. Mouse RAW264.7 cells were incubated with 10 μM RNase3 and/or 100 nM of Bafilomycin A1. Primary anti-LC3 antibody and Alexa-488 labeled secondary antibody were used to detect LC3 (green). Nuclei were labeled with DAPI (blue). Following immunostaining, 100 cells from each treatment were analyzed and the number of LC3 puncta per cell was calculated by Image J. *Statistically significant differences are indicated, p < 0.05.