Table 1.
Comparison of the antimycobacterial activity of RNase3, RNase6, and RNase7 and their respective active center mutants (H15A).
| Protein | Extracellular MBC (μM) | MAC (μM) | Intracellular MIC (μM) | IC50 (μM) | ||||
|---|---|---|---|---|---|---|---|---|
| M. aurum | M. smegmatis mc2155 | M. bovis BCG | M. aurum | M. smegmatis mc2155 | M. bovis BCG | M. aurum | RAW264.7 | |
| RNase3 | 18.75 ± 0.05 | 9.37 ± 0.05 | 18.75 ± 0.05 | 2.34 ± 0.5 | 1.17 ± 0.3 | 4.68 ± 1.0 | 5 ± 0.05 | > 25 |
| RNase3-H15A | 18.75 ± 0.05 | 18.75 ± 0.05 | 18.75 ± 0.05 | 4.68 ± 0.5 | 4.68 ± 1.0 | 4.68 ± 1.0 | 10 ± 0.05 | > 25 |
| RNase6 | 18.75 ± 0.05 | 18.75 ± 0.05 | 18.75 ± 0.05 | 4.68 ± 1.0 | 2.34 ± 0.5 | 4.68 ± 1.0 | 10 ± 0.05 | > 25 |
| RNase6-H15A | 18.75 ± 0.05 | 18.75 ± 0.05 | 37.5 ± 0.05 | 4.68 ± 1.0 | 2.34 ± 0.5 | 4.68 ± 1.0 | 10 ± 0.05 | > 25 |
| RNase7 | 18.75 ± 0.05 | 18.75 ± 0.05 | 18.75 ± 0.05 | > 10 | > 10 | > 25 | 10 ± 0.05 | > 25 |
| RNase7-H15A | 18.75 ± 0.05 | 18.75 ± 0.05 | 18.75 ± 0.05 | > 10 | > 10 | > 25 | 10 ± 0.05 | > 25 |
Extracellular minimum bactericidal concentration (MBC100) and intracellular minimum inhibitory concentration (MIC100) were calculated at 72 h incubation time using the SPOTi assay. Minimum agglutination concentration (MAC) and Inhibitory Concentration (IC50) for RAW 264.7 mouse macrophages were assayed as described in the methodology. Results are an average of three independent repeated experiments. Mean average values ± SD are indicated.