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. 2019 Jul 8;4:24. doi: 10.1038/s41541-019-0121-9

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© This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Electron microscopy of OMPC and Pfs230-OMPC conjugate. a Transmission electron microscopic image of OMPC. b Transmission electron microscopic image of Pfs230-OMPC-3 stored at 4 °C for one year, after incubation with anti-Pfs230 antibody, 4F12 (1:10), followed by gold-labeled secondary antibody. c Transmission electron microscopic image of Pfs230-OMPC-3 incubated with gold-labeled secondary alone (omitting incubation with primary antibody, 4F12). Images were taken on a FEI Biotwin Tecnai microscope and collected on an AMT XR611 camera system. Scale bar: 100 nm