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. 2019 Jul 8;10(7):524. doi: 10.1038/s41419-019-1754-3

Fig. 3. The cell death and mitochondrial damage were further exacerbated in podocytes of Grn−/− diabetic mice.

Fig. 3

a In situ terminal deoxynucleotidyl transferase-mediated UTP nick-end labeling (TUNEL) assays were performed to assess the cell death in glomerulus. Nuclei were revealed using 4′,6-diamidino-2-phenylindole (DAPI) staining. Quantitative assessment of the number of cell death (number of TUNEL-positive cells per glomerulus). b Double immunofluorescence staining for cleaved-caspase 3 (green) and synaptopodin (red) in the kidney from different groups of mice. Quantitative assessment of the number of cleaved-caspase 3-positive podocytes in glomerulus. c Representative TEM images of glomeruli demonstrating mitochondrial morphology in podocytes of kidney sections and quantification of percentage of altered mitochondria characterized by mitochondria swelling, vacuolization and cristae fragmentation in podocytes from different groups of mice. Red arrows indicate representative mitochondria. d Representative photomicrographs and semiquantitation of cytochrome C oxidase (complex IV) subunit IV isoform 1 (COXIV) immunohistochemical staining in the kidney from different groups of mice. *P < 0.05 vs. sham-operated mice, #P < 0.05 vs. WT diabetic mice (n = 6)