Skip to main content
. 2019 Jul 8;10(7):523. doi: 10.1038/s41419-019-1730-y

Fig. 1. CdCl2 induces necroptosis in liver tissues of ICR mice.

Fig. 1

The ICR mice were intraperitoneally injected with CdCl2 (1 mg/kg, n = 3) or physiological saline (Ctrl group, n = 3) every day for one week. a Body weight curve. b Histological changes (HE staining) of liver. Arrow indicates the histological changes (hepatic cord incomplete and vacuolation) of the liver. c The levels of necroptosis-related proteins (RIPK1, RIPK3, MLKL, and p-MLKL) were analyzed by Western blot. d, e Liver tissues were subjected to immunohistochemistry (IHC) using p-MLKL antibody. d The representative images of IHC were shown. Arrow indicates the p-MLKL signals in the liver. The scale bar is 50 μm. e The images were semi-quantified using ImagePro-plus 6.0. Five fields of view were calculated for each group. Data are expressed as the mean ± standard deviation (SD). P < 0.05, *significantly different from Ctrl