Fig. 6. Inhibition of Drp1 counteracts mitochondrial fission and RB translocation to mitochondria induced by CdCl₂ in L02 cells.

a-d L02 cells were pretreated with Mdivi-1 (10 μM) for 30 min and then treated with CdCl₂ (20 μM) for another 6 h. a Whole cell lysates and b cytoplasmic (Cyto) and mitochondrial (Mito) fractions of L02 cells in four groups were analyzed by Western blot with the indicated antibodies. c L02 cells were subjected to IF staining with MitoTracker (red) and anti-RB antibody (green). DAPI stained the nuclei. The scale bar is 10 μm. d Pearson’s correlation for the co-localization of mitochondria with RB is in bar graphs. Red-green overlap sections were quantified using ImagePro-plus 6.0. P < 0.05, *significantly different from Ctrl, ^#significantly different from CdCl₂ treatment. e-g Drp1 silencing with siDNM1L decreased Cd-induced mitochondrial fission and RB expression. siNC is the negative Ctrl of siRNA. e Whole cell lysates of L02 cells were analyzed by Western blot with the indicated antibodies. f L02 cells were subjected to IF staining with MitoTracker (red), anti-RB antibody (green), and DAPI (blue, staining nuclei). The scale bar is 10 μm. g Mito/RB signal with red-green overlap sections were quantified using ImagePro-plus 6.0. Ten fields of view were calculated for each group. Data are expressed as the mean ± standard deviation (SD). P < 0.05, *significantly different from Ctrl or siNC, ^#significantly different from CdCl₂ group