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. 2019 Jul 2;10:1461. doi: 10.3389/fmicb.2019.01461

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Copyright © 2019 Gong, Liu, Huang, Hao, Li and Sun.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of NBP on the Δψ_m of C. albicans. Yeast cells pretreated with diverse concentrations of NBP (0, 32, 64, and 128 μg/ml) for 4 h were incubated with 15 μM Rh123 and then analyzed by a flow cytometer. (A–D) Effect of different doses of NBP on Δψ_m loss in C. albicans. (A) Negative control, (B) 64, (C) 128, (D) 256 μg/ml. Representative images from three independent experiments are shown. The percentages of cells in the left section of the histogram refer to the number of cells with depolarized mitochondria. (E) Effect of NBP on the mean fluorescence intensity of Rh123-stained C. albicans. ^***p < 0.001 compared with the control group (one-way ANOVA).