Table 3.
Drugs interactions of NBP and FLC against preformed C. albicans biofilms in vitro.
| Straina | Time(h)b | sMIC80(μg/ml)c | FICIc | Interpretationd | |||
|---|---|---|---|---|---|---|---|
| Alone | Combined | ||||||
| NBP | FLC | NBP | FLC | ||||
| CA4 | 4 | 128 | >1,024 | 64 | 0.5 | >0.5 | NI |
| 8 | 128 | >1,024 | 64 | 0.5 | >0.5 | NI | |
| 12 | 128 | >1,024 | 64 | 4 | >0.5 | NI | |
| 24 | >512 | >1,024 | >512 | >256 | 1.3 | NI | |
| CA10 | 4 | 128 | >1,024 | 32 | 0.5 | 0.25 | SYN |
| 8 | 128 | >1,024 | 32 | 4 | 0.26 | SYN | |
| 12 | 256 | >1,024 | 64 | 8 | 0.27 | SYN | |
| 24 | >512 | >1,024 | >512 | >1,024 | 2 | NI | |
| CA16 | 4 | 128 | >1,024 | 32 | 0.5 | 0.25 | SYN |
| 8 | 128 | >1,024 | 32 | 2 | 0.25 | SYN | |
| 12 | 256 | >1,024 | 64 | 4 | 0.26 | SYN | |
| 24 | >512 | >1,024 | >512 | >1,024 | 2 | NI | |
a
CA, Candida albicans.
b
Time indicates incubation period of preformed biofilms.
c
NBP, n-butylphthalide; FLC, fluconazole; FICI, fractional inhibitory concentration index.
d
SYN, synergism; NI, no interaction.
sMIC80 denotes sessile minimum inhibitory concentration of drug that produced an 80% reduction of biofilms metabolic activity compared with the growth control. sMIC80 values and FICIs are shown as the median of three independent experiments.