Fig. 2. Apogossypol-mediated ER membrane reorganisation antagonises mitochondrial fission.

H1299 cells were exposed to apogossypol (20 μΜ) for 1 h, followed by either a DMSO, b A-1210477 (10 µM) for 4 h or c CCCP (20 μΜ) for 1 h and assessed for mitochondrial membrane integrity and ER membrane reorganisation by immunostaining with HSP60 and RTN4 antibodies, respectively. Scale bar: 10 μm. The boxed regions in the images are enlarged on the right to show mitochondrial structural changes in the indicated cells. Quantification of mitochondrial fission was performed by counting ~100 cells from 3 independent experiments. Error bars = Mean ± SEM. ***p ≤ 0.001. d Western blot analysis of whole cell lysates of H1299 cells exposed to apogossypol (20 μΜ) in the presence and absence of A-1210477 or CCCP