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. 2019 Jul 8;9:9820. doi: 10.1038/s41598-019-46088-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CTGF is directly targeted by miR-212-3p via the 3′-UTR. (A) Schematic of putative miR-212-3p binding sequences in the 3′-UTR of CTGF, and the generation of CTGF mutants. Relative (B) mRNA and (C) protein expression of CTGF in HCCLM3 or Huh7 cells with or without miR-212-3p were determined. (D) Luciferase assays were used to determine reporter activity in HCCLM3 or Huh7 cells co-transfected with either miR-212-3p mimics or NC, and either pmirGLO-CTGF-WT or pmirGLO-CTGF-MUT. (E) CTGF mRNA expression in the HCC samples was determined by qRT-PCR and (F) negatively correlated with miR-212-3p expression. *P < 0.05; **P < 0.01; ***P < 0.001.