Fig. 2. Rpph1 (ribonuclease P RNA component H1) regulated inflammation and mesangial cell (MC) proliferation.

a The expression levels of monocyte chemoattractant protein-1 (Mcp-1) and tumor necrosis factor-α (Tnf-α) in the renal tissue of db/db diabetic nephropathy (DN) mice (n = 3 for 12 weeks) and normal controls (n = 3 for 12 weeks) were assessed by immunohistochemistry and quantitative analysis. b Messenger RNA (mRNA) expression levels of Mcp-1 and Tnf-α in the renal tissue of db/db DN mice and normal controls were assessed by quantitative real-time PCR (qRT-PCR). c Twenty-four hours after transfection of Rpph1 over-expression plasmid in L-MC, or Rpph1 small interfering RNA (siRNA) in H-MC, the expression levels of Mcp-1 mRNA and Tnf-α mRNA were detected by qRT-PCR. d Forty-eight hours after transfection of Rpph1 siRNA in H-MC, or over-expression plasmid in L-MC, the expression levels of Mcp-1 and Tnf-α were assessed by enzyme- linked immunosorbent assay (ELISA). e Forty-eight hours after transfecting Rpph1 siRNA in H-MC, or over-expression plasmid in L-MC, the expression levels of Mcp-1 and Tnf-α were assessed by Western blot and quantitative analysis. f Forty-eight hours after transfecting Rpph1 siRNA in H-MC, or over-expression plasmid in L-MC, the expression levels of Mcp-1 and Tnf-α were detected by immunofluorescence. g Forty-eight hours after transfecting Rpph1 siRNA in H-MC, or over-expression plasmid in L-MC, cell proliferation rates were detected by EdU (5-ethynyl-2′-deoxyuridine) and quantitative analysis. In all panels, the data are representative of three independent experiments. Data are presented as mean ± SD. *P < 0.05, **P < 0.01, NS not significant