Fig. 3.

CPUY192018 enhanced the antioxidant capacity in HK-2 cells. (A) Western blot analysis of the proteins SOD1 and GPx2 in human HK-2 cells after treatment with various concentrations (0, 0.1, 1, 10 μM) of CPUY192018 for 10 h. The activities of SOD (B), GSH-Px (C), CAT (D) and MDA (G) were measured in HK-2 cells. (E) The ratio of GSH/GSSG were measured in HK-2 cells. (F) Living Cell Microscopy. HK-2 cells were pretreated with 10 μM CPUY192018 for 10 h and then exposed to LPS (200 ng/mL) for an additional 6 h. After this treatment, the cells were stained with 10 μM cH₂DCF-DA for 20 min at 37 °C and living cell fluorescence microscopy was performed. The values shown are the means ± SEM (n = 3 independent observations). ***P < 0.001, **P < 0.01 and *P < 0.05, one-way ANOVA with Tukey–Kramer posttest.