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. 2019 Jul 2;10:1489. doi: 10.3389/fmicb.2019.01489

FIGURE 2.

FIGURE 2

YneP heterologously expressed in E. coli synthesizes a variety of methylketone with medium chain length and functions as a methylketone synthase. (A) SDS-PAGE confirmed expression of the recombinant proteins of YtpA and YneP in E. coli BL21. Lane M represented the protein Marker; Lane 1–7 showed the negative control of E. coli BL21 with blank vector pET28a, E. coli BL21 overexpressing yneP, the supernatant of E. coli BL21 containing yneP, the precipitate of E. coli BL21 containing yneP, E. coli BL21 overexpressing ytpA, the supernatant of E. coli BL21 containing ytpA, the precipitate of E. coli BL21 containing ytpA, respectively. Arrowhead and arrow represented the proteins of YneP and YtpA expressed in E. coli BL21, respectively. (B) GC-MS analyses of the VOCs produced by the negative control E. coli BL21 with blank vector, E. coli BL21 with YtpA overexpression and E. coli BL21 with YneP overexpression. (C) GC-MS analyses of the VOCs produced by B. nematocida B16 strain.