Table 1.
Strains and plasmids used in this study.
| Strain or plasmid | Genotype/description | Source or references |
|---|---|---|
| Strains | ||
| B. nematocida B16 | Wild Type | Laboratory stock |
| B. subtilis 168 | Wild Type | Laboratory stock |
| E. coli DH5α | Clone strain | TaKaRa |
| E. coli BL21 | E. coli strain for heterologously expressing protein | Laboratory stock |
| E. coli EyneP | BL21 heterologously expressing yneP of B. nematocida | This study |
| E. coli EytpA | BL21 heterologously expressing ytpA of B. nematocida | This study |
| E. coli EyneP’ | BL21 heterologously expressing yneP of B. subtilis | This study |
| B. subtilis EyneP’ | B.subtilis 168 homologously expression yneP of B. subtilis | This study |
| BSP1 | B.subtilis 168 amyE::PyneP-627bp-lacZ(Cmr) | This study |
| BSP2 | B.subtilis 168 amyE::PyneP-518bp-lacZ(Cmr) | This study |
| BSP3 | B.subtilis 168 amyE::PyneP-383bp-lacZ(Cmr) | This study |
| BSP4 | B.subtilis 168 amyE::PyneP-356bp-lacZ(Cmr) | This study |
| BSP5 | B.subtilis 168 amyE::PyneP-217bp-lacZ(Cmr) | This study |
| BSP6 | B.subtilis 168 amyE::PyneP-198bp-lacZ(Cmr) | This study |
| BNP1 | B.nematocida B16 amyE::PyneP-628bp-lacZ(Cmr) | This study |
| BNP2 | B.nematocida B16 amyE::PyneP-491bp-lacZ(Cmr) | This study |
| BNP3 | B.nematocida B16 amyE::PyneP-444bp-lacZ(Cmr) | This study |
| BNP4 | B.nematocida B16 amyE::PyneP-229bp-lacZ(Cmr) | This study |
| BNP5 | B.nematocida B16 amyE::PyneP-205bp-lacZ(Cmr) | This study |
| BNP6 | B.nematocida B16 amyE::PyneP-200bp-lacZ(Cmr) | This study |
| Plasmids | ||
| pMD-19T | Cloning vector in E. coli | TaKaRa |
| pMD-18T | Cloning vector in E. coli | TaKaRa |
| pET-28a | Expressional vector in E. coli BL21 | Laboratory stock |
| pET28a-yneP | Recombinant expressional plasmid for expressing yneP of B. nematocida | This study |
| pET28a-ytpA | Recombinant expressional plasmid for expressing ytpA of B. nematocida | This study |
| pET28a-yneP’ | Recombinant expressional plasmid for expressing yneP’ of B. subtilis | This study |
| pIs284 | Insertion vector to amyE, chloramphenicol resistance, lacZ | From Mitsuo Ogura |
| pDG148 | Expressional vector in B. subtilis | From Francois Denizot |
| pDG148-yneP’ | Expressional plasmid for homologously expressing yneP in B. subtilis | This study |
| pCP115 | Gene knockout plasmid in Bacillus sp. | Bacillus Genetic Stock Center |
| pCP115-yneP | yneP gene knockout plasmid in B. nematocida | This study |