Table 2.
The primers used in this study.
| Primer | Nucleotide sequence (5′→3′) | Functions |
|---|---|---|
| FytpA | GGATCCATGTGGACTTGGAAAGCAG | pET28a-ytpA |
| RytpA | CTCGAGTCAAATATACTGATCTGTAAAG | pET28a-ytpA |
| FyneP | GGATCCTTGCATGTGTCAAAAAAAG | pET28a-yneP |
| RyneP | CTCGAGTTATTTTTTTGCCTTTTCGT | pET28a-yneP |
| FyneP’ | GGATCCTTGCATGTGTCAAAAAAAG | pET28a-yneP’ |
| RyneP’ | CTCGAGCTATTTTTTGGCCTTTTCAT | pET28a-yneP’ |
| FbsyneP’ | GTCGACTTGCATGTATCAAAAAAA | pDG148-yneP’ |
| RbsyneP’ | GCATGCCTAGTGGTGATGGTGATGATGTTTTTTGGCCTTTTCATAT | pDG148-yneP’ |
| BSP1 | GAATTCCATTATCACCTCAATCAT | Reporter gene upstream 1 |
| BSP2 | GAATTCATAAAAACATTGATATTTAC | Reporter gene upstream 2 |
| BSP3 | GAATTCAACTGCAGCAAAAGAC | Reporter gene upstream 3 |
| BSP4 | GAATTCGAGAAAAACGTTTACTAC | Reporter gene upstream 4 |
| BSP5 | GAATTCGGCTTCGTTATCAAAAA | Reporter gene upstream5 |
| BSP6 | GAATTCAACACGTGGAAAATTTG | Reporter gene upstream 6 |
| BSPRV | GGATTCTAACGAGATCAACAGGAA | Reporter gene downstream |
| BNP1 | GAATTCTCACATGATTGGCTTTCC | Reporter gene upstream 1 |
| BNP2 | GAATTCTAAAAACATTGATATTTACTTATG | Reporter gene upstream 2 |
| BNP3 | GAATTCTGTTTTGCGTCAGGTAGA | Reporter gene upstream 3 |
| BNP4 | GAATTCTTGTGAGAAAATTGTGATGG | Reporter gene upstream 4 |
| BNP5 | GAATTCTTCTATTAAGGTTCTTTTGG | Reporter gene upstream 5 |
| BNPRV | GGATTCTTTATCAGGGTCTCCGCCGA | Reporter gene downstream |
The underlined sequences mean the restriction enzyme cutting sites.