Table 3.
MS analysis and yields of type B peptides before and after nisin part removal considering the highest dehydration number possible.
| Name | Predicted mass (Da) | Measured mass (Da) | Observed dehydrations/total possible dehydrations | Yield (μg/L) |
|---|---|---|---|---|
| Full length type B fusion peptides | ||||
| pNZE-nis(Δ23-34)-G-VSP | 5562.71 | 5560.97 | 6/6 | 660 |
| pNZE-nis(Δ23-34)-ASPRG-VSP | 5955.94 | 5955.60 | 7/7 | 570 |
| pNZE- nis(Δ23-34)-EG-VSP | 5691.76 | 5691.56 | 6/6 | 760 |
| pNZE- nis(Δ23-34)-NG-VSP | 5676.75 | 5676.39 | 6/6 | 530 |
| pNZE- nis(Δ23-34)-MG-VSP | 5693.75 | 5693.35 | 6/6 | 620 |
| pNZE- nis(Δ23-34)-WG-VSP | 5748.79 | 5748.39 | 6/6 | 610 |
| Vasopressin released after cleavage of type B peptides | ||||
| pNZE-nis(Δ23-34)-G-VSP | 1110.21 | 1112.68 | 1/1 | 30 |
| pNZE-nis(Δ23-34)-ASPRG-VSP | 1110.21 | 1111.89 | 1/1 | 10 |
| pNZE- nis(Δ23-34)-EG-VSP | 1110.21 | 1111.63 | 1/1 | 100 |
| pNZE- nis(Δ23-34)-NG-VSP | 1110.21 | 1111.44 | 1/1 | 38 |
| pNZE- nis(Δ23-34)-MG-VSP | 1110.21 | 1110.91 | 1/1 | 54 |
| pNZE- nis(Δ23-34)-WG-VSP | 1110.21 | 1111.87 | 1/1 | 36 |