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. 2019 Jul 8;39(7):BSR20180551. doi: 10.1042/BSR20180551

Figure 5. LIFR-AS1 up-regulates Sufu expression in breast cancer cells by sponging miR-197-3p.

Figure 5

(A) A putative binding site of miR-197-3p in the 3′UTR of Sufu was predicted by miRanda algorithms. (B) Luciferase activity was examined after co-transfection of miR-197-3p and wild-type Sufu 3′UTR fragment or mutant Sufu 3′UTR fragment. (C) Sufu protein expression was examined by Western blotting after treatment with negative control or miR-197-3p mimics. (D) Luciferase activity was determined after co-transfection of siLIFR-AS1 and wild-type Sufu 3′UTR fragment or mutant Sufu 3′UTR fragment. (E) Sufu protein expression was examined after treatment with negative control siRNA or siLIFR-AS1. ***P<0.001.