Figure 3.

Significant reduction in sperm numbers and motility in the conditional Ift74 KO mice. (A) Western blot analysis of testicular IFT74 protein expression in control and conditional Ift74 KO mice. Note that IFT74 protein was missing in the conditional KO mice. Epididymal sperm were collected and physiologic parameters were compared between the control and conditional Ift74 KO mice. In the conditional Ift74 KO mice, there are significant reductions in sperm count (B), percentage of motile sperm (C), and sperm motility (D). Data are expressed as the Means ± SD (n = 4). *P < 0.05 compared with the control mice.